diff --git a/homeassistant/components/otbr/manifest.json b/homeassistant/components/otbr/manifest.json
index f4029f4aa9e2..bc7d62cba032 100644
--- a/homeassistant/components/otbr/manifest.json
+++ b/homeassistant/components/otbr/manifest.json
@@ -8,5 +8,5 @@
   "documentation": "https://www.home-assistant.io/integrations/otbr",
   "integration_type": "service",
   "iot_class": "local_polling",
-  "requirements": ["python-otbr-api==2.7.0"]
+  "requirements": ["pyserial==3.5", "python-otbr-api==2.7.0"]
 }
diff --git a/requirements_all.txt b/requirements_all.txt
index 7be491a2b029..c4ae19783e38 100644
--- a/requirements_all.txt
+++ b/requirements_all.txt
@@ -2334,6 +2334,7 @@ pyserial-asyncio-fast==0.16
 
 # homeassistant.components.acer_projector
 # homeassistant.components.crownstone
+# homeassistant.components.otbr
 # homeassistant.components.usb
 # homeassistant.components.zwave_js
 pyserial==3.5
diff --git a/requirements_test_all.txt b/requirements_test_all.txt
index 13d55c4810b7..fcbf995ebaf8 100644
--- a/requirements_test_all.txt
+++ b/requirements_test_all.txt
@@ -1943,6 +1943,7 @@ pysensibo==1.2.1
 
 # homeassistant.components.acer_projector
 # homeassistant.components.crownstone
+# homeassistant.components.otbr
 # homeassistant.components.usb
 # homeassistant.components.zwave_js
 pyserial==3.5